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RGUHS MBBS Biochemistry March 2022 (1025 CBME)

1025Rajiv Gandhi University of Health Sciences · Biochemistry · 2022 · March · CBME

Questions (30)

  1. 1.A child of two years presented with convulsions. He was taking normal feeds. But his mother observed mousy odour of child’s urine. Urine ferric chloride test was positive. Plasma phenylalanine was found to be elevated. He was diagnosed to have phenyl Ketonuria a) What is the biochemical defect in phenyl Ketonuria? b) Describe the catabolism of phenylalanine c) Give the biochemical reasons for mousy odour in this case
    10 Marks
  2. 2.Describe protein biosynthesis in prokaryotes. Mention three inhibitors of protein biosynthesis
    10 Marks
  3. 3.A 45 year old man presented with severe back pain and weakness. He had lost 7 kgs in last three months. He complained of loss of appetite and fatigue. X-ray showed punched out lesions. Urine is positive for Bence Jones protein a) Name the disorder in above case b) What are Bence Jones proteins? c) What is the electrophoretic pattern that is observed in this case?
    5 Marks
  4. 4.A known case of diabetes and hypertension on irregular treatment presented with swelling of both lower limbs and puffiness of face. He was diagnosed with chronic kidney disease (CKD) a) Mention two biochemical parameters altered in CKD. b) Explain the test to assess glomerular function of Kidney.
    5 Marks
  5. 5.What is Porphyria? Write the types of porphyria. Mention enzyme defect and clinical features of acute intermittent porphyria
    5 Marks
  6. 6.How are free radicals generated? Describe the free radical scavenging systems
    5 Marks
  7. 7.List the hormones which function with cAMP as second messenger. Describe the mechanism of action of steroid hormones
    5 Marks
  8. 8.Enumerate the reactions of purine salvage pathway. Name the disorders that is associated with this pathway and write its clinical features
    5 Marks
  9. 9.What are restriction endonucleases? Give two examples. Explain their role in Recombinant DNA technology
    5 Marks
  10. 10.What is polymerase chain reaction (PCR)? Explain the steps in PCR. Write two applications of PCR
    5 Marks
  11. 11.List out the assays done for evaluation of thyroid function. Enumerate the laboratory findings in hyperthyroidism
    3 Marks
  12. 12.What is detoxification? Give two examples of detoxification by oxidative reaction
    3 Marks
  13. 13.What are reference intervals? List out two pre analytical variables
    3 Marks
  14. 14.What is Southern Blotting Technique? Mention its applications
    3 Marks
  15. 15.Name three nucleotides and write their biological importance
    3 Marks
  16. 16.What is innate and adaptive immune response?
    3 Marks
  17. 17.Give three examples of tumour markers and write their clinical importance
    3 Marks
  18. 18.Write the serum and urinary biochemical findings in hemolytic jaundice
    3 Marks
  19. 19.What are oncogenes? What are the factors that activate oncogenes?
    3 Marks
  20. 20.What are essential amino acids? Give examples
    3 Marks
  21. 21.Heme is converted to bilirubin in A. Mitochondria B. Microsomes C. Golgi apparatus D. Plasma membrane
    1 Mark
  22. 22.Histones are rich in A. Glutamic acid and aspartic acid B. Glycine and serine C. Methionine and cysteine D. Arginine and Histidine
    1 Mark
  23. 23.Which of the following is a termination codon? A. AAG B. CAG C. CAA D. UAG
    1 Mark
  24. 24.The amino acid which do not allow formation of alpha helix is A. Glutamate B. Proline C. Tyrosine D. Histidine
    1 Mark
  25. 25.Immunoglobulin which crosses placenta is A. IgG B. IgA C. IgM D. IgD
    1 Mark
  26. 26.Which of the following compound used in the treatment of gout is a competitive inhibitor of xanthine oxidase? A. Probenicid B. Allopurinol C. Colchicine D. Acetaminophen
    1 Mark
  27. 27.Normal serum level of albumin is A. 1.5 to 2.5 gm/dl B. 3.5 to 5 gm /dl C. 6 to 7.5 gm/dl D. 9 to 11 gm/dl
    1 Mark
  28. 28.End product of catabolism of protein is? A. Urea B. Uric acid C. Creatinine D. Orotic acid
    1 Mark
  29. 29.Which of the following antioxidant enzyme is selenium dependent? A. Glutathione peroxidase B. Glutathione reductase C. NADPH oxidase D. Catalase
    1 Mark
  30. 30.In which of the following phase of cell cycle DNA replication takes place A. G1 B. G2 C. S D. M
    1 Mark

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