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RGUHS MBBS Biochemistry February 2023 (1025 CBME)

1025Rajiv Gandhi University of Health Sciences · Biochemistry · 2023 · February · CBME

Questions (30)

  1. 1.A 10 year old boy was brought to the hospital for self-multilating behaviour and delayed development. His serum uric acid level was 15 mg/dl. A provisional diagnosis of Lesch Nyhan syndrome was made. a) Write the biological reference interval for serum uric acid level. b) Name the enzyme defect and the pathway affected in the above disorder. c) Which tissues are dependent on this pathway? d) Enumerate the other causes of hyperuricemia e) What is the role of allopurinol in the treatment of hyperuricemia?
    10 Marks
  2. 2.Describe the steps of DNA replication in prokaryotes. List the difference between prokaryotic and eukaryotic DNA replication.
    10 Marks
  3. 3.a) A 15 year old tribal boy was admitted with complaints of fatigue, recurrent fever and pain in arms and legs. His peripheral blood smear shows sickled erythrocytes and a hemoglobin concentration of 6 gm%. a) Name the molecular defect in this disorder. b) Explain the mechanism of sickling. c) What is the cause of anemia in this condition?
    5 Marks
  4. 4.d) An intravenous drug user was found to have developed jaundice. Laboratory investigations revealed hepatitis B infection. His total serum bilirubin was 10.2 mg/dl. a) What type of jaundice is seen in above case? b) Explain van den Bergh test and write its interpretation in the above case.
    5 Marks
  5. 5.Describe the steps in this polymerase chain reaction (PCR). Name the type of PCR used in the diagnosis of Covid 19.
    5 Marks
  6. 6.Name any three products derived from tyrosine. Describe the metabolic pathway leading to the formation of any one of the product from tyrosine.
    5 Marks
  7. 7.Draw the structure of immunoglobulin G and label it. Enumerate its functions.
    5 Marks
  8. 8.What is lipid peroxidation? Explain the role of antioxidants in scavenging reactive oxygen species.
    5 Marks
  9. 9.Write the steps in the formation of creatine and glutathione from glycine.
    5 Marks
  10. 10.Describe the glomerular function tests.
    5 Marks
  11. 11.Name three tumor markers and mention their significance.
    3 Marks
  12. 12.What is the enzyme defect in maple syrup urine disease? What are its characteristic features?
    3 Marks
  13. 13.List the adrenal function tests. Mention one disorder associated with abnormal adrenal function.
    3 Marks
  14. 14.Enlist three biologically important peptides and write their functions.
    3 Marks
  15. 15.What are xenobiotics? Write two detoxification reactions by conjugation.
    3 Marks
  16. 16.Name three biologically important nucleotides and mention their importance.
    3 Marks
  17. 17.Mention three advantages of automation in clinical biochemistry laboratory.
    3 Marks
  18. 18.Enlist three post-translational modifications with suitable examples.
    3 Marks
  19. 19.Mention normal albumin/globulin (A/G) ratio. Write two conditions where A/G ratio is altered.
    3 Marks
  20. 20.What is restriction fragment length polymorphism (RFLP). Mention two clinical applications of RFLP.
    3 Marks
  21. 21.All the following are glucogenic amino acids EXCEPT A. Valine B. Phenylalanine C. Glycine D. Leucine
    1 Mark
  22. 22.N3 of purine ring is donated by A. Asparate B. Glycine C. Glutamine D. Ammonia
    1 Mark
  23. 23.Which of the following enzymes is responsible for immortality of cancer cells? A. Telomerase B. RNA polymerase I C. DNA polymerase D. Helicase
    1 Mark
  24. 24.In which of the following conditions is serum direct bilirubin elevated A. Physiological jaundice in the newborn B. Crigler – Najjar syndrome C. Gilbert’s syndrome D. Gall stones
    1 Mark
  25. 25.Enzyme defect in acute intermittent porphyria is A. ALA synthase B. ALA dehydratase C. PBG deaminase D. Uroporphyrinogen decarboxylase
    1 Mark
  26. 26.Closeness of a result to the true value is termed as A. Precision B. Accuracy C. Sensitivity D. Specificity
    1 Mark
  27. 27.Reference interval for pH of urine is A. 3.5 to 4.5 B. 5.5 to 6.5 C. 7.5 to 8.5 D. 9.5 to 10.5
    1 Mark
  28. 28.Southern blotting technique is used for detection of A. DNA B. RNA C. Protein D. Lipoprotein
    1 Mark
  29. 29.Which of the following is an example for acute phase protein? A. Prothrombin B. C-reactive protein C. Lipoprotein A D. Thyroxine binding globulin (TBG)
    1 Mark
  30. 30.Epidermal growth factor (EGF) is produced by A. Platelets B. Monocytes C. Endothelial cells D. Fibroblasts
    1 Mark

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